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Cell culture
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Last updated on 2021-12-07.

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Juan C. Sanchez-Arias

Trained as medical doctor and neuroscientist, I’m currently investigating the molecular and cellular mechanisms underlying cardiac arrythmias and seizures associated with a novel genetic variant in an ion channel adapter protein combinng model organism research, clinical genetics, bio-informatics, and computational modelling.

Education

PhD in Neuroscience

University of Victoria

Victoria, BC, Canada

2020 - 2015

  • Dissertation: Pannexin 1 regulates dendritic spine formation.
  • Relevant coursework: Developmental neurobiology, Tools for the study of ion channels.
  • GPA: 8.2/9.0.

Doctor of Medicine (Medico y Cirujano)

Universidad del Valle - School of Medicine

Cali, Valle, Colombia

2014 - 2007

  • Pre-Diploma Rotatory Internship: Hospital Universitario del Valle ESE - Universidad del Valle. Cali, Valle. Colombia.
  • Research Internship: Centro de Estudios Cerebrales (Centre for Brain Studies). Universidad del Valle. Cali, Valle. Colombia.
  • Observership: Functional Neurosurgery. Department of Neurosurgery. University of Illinois Hospital & Health Sciences System. University of Illinois at Chicago. Chicago, IL. USA. Supervisor: Dr. Konstantin Slavin.
  • Professional elective: Neurosurgery and Neurocritical Care. Department of Neurosurgery.Hospital Universitario del Valle ESE - Universidad del Valle. Cali, Valle. Colombia.
  • Relevant Coursework: Functional Neuroanatomy, Cerebral cortex cytoarchitectonics, Scientific Integrity, Biostatistics, Addiction and Pharmacology, Systems Pathology.

Research Experience

Postdoctoral Fellow

Division of Medical Sciences

University of Victoria

Current - 2020

  • Advisors: Leigh Anne Swayne, PhD; Laura Arbour MSc, MD,
  • Area of study: ankyrin adapter proteins, ion channels, calcium and voltage dysregulation in caridomyocytes and neurons with ankyrin variants.
  • Community medical genetics in inherited arrhythmias.
  • Cardiomyocyte and neuronal calcium homoestasis
  • Bioinformatics and computational modelling of cardiomyocte physiology.

Graduate Research Assistant

Division of Medical Sciences

Univesity of Victoria

2020 - 2015

  • Advisor: Leigh Anne Swayne, PhD.
  • Area of study: Pannexin 1 channels, dendritic spine plasticity, synapse formation, channel trafficking, neuronal cytoskeleton dynamics, neural stem cells, advanced microscopy for cell biology.
  • Generated conditional and conditional-inducible knockout models for the study of cerebral cortex development.
  • Optimized protocols to generate primary neuronal cultures from neonatal mice suitable for network analysis.
  • Developed methods to visualize dendritic spines and filopodia in tissue sections and living primary neurons.

Research Medical Intern

Centro de Estudios Cerebrales

Universidad del Valle

2014 - 2013

  • Advisors: Martha Escobar, MSc; Hernan Pimienta, MSc, Efrain Buritica, PhD.
  • Area of study: functional neuroanatomy, cerebral cortex organization, traumatic brain injury, stroke, neuroprotection.
  • Performed carotid ligation in Whistar rats using microsurgery techniques.
  • Investigated diffuse traumatic brain injury using a weight-drop model, organotypical rat rain brain slices. fluorescent immunochemistry and microscopy.

Undergraduate Medical Researcher

School of Public Health

Universida del Valle

2011 - 2010

  • Advisors: Enrique A. Estevez, MD and Elsa P. Muñoz, MD, MPH
  • Area of study: ardiovascular risk factor assessment in spinal cord injured patient assisting to a tertiary-level hospital.
  • Prepared a research project proposal, liaised with ethical boards, and established a network of collaborators to complete a standarized and regional survey in cardiovascular health.
  • Led data managament and prepared reports and presentations to share project results in local and national meetings.
  • Contributed to the assessment and management of patients with chronic spinal cord injury, including prescribing rehabilitation plans and ordering of radiological and laboratory ancillary tests. Discussed cases with Physical Medicine and Rehabilitation attendings and residents.

Research interests: Translational research, bio-image analysis, neuronal and cardiac physiology, bioinformatics, clinical genetics, open science.




Teaching Experience

”Any man could, if he were so inclined, be the sculptor of his own brain.” Santiago Ramon y Cajal

Foundations of Medical Pracitce I and II

Island Medical Program

University of Victoria

2020 - 2017

  • Courses: MEDD412 (Year 1), MEDD421 (Year 2), MEDD422 (Year 2).
  • TA for Neuroanatomy Bootcamp and Laboratories.
  • Subjects: Cranial Nerves V & VII and Pain, Eye Movements and Brainstem, Cerebral Cortex, Functional Areas, and Blood Supply, Control of Movement and Cerebellum, Limbic System/Dementia

Selected Publications, Posters, and Talks

A systematic, open-science framework for quantification of cell-types in mouse brain sections using fluorescence microscopy

Frontiers in Neuroanatomy

N/A

2021 - 2021

  • Juan C. Sanchez-Arias, Micael Carrier, Simona D. Frederiksen, Olga Shevtsova, Chloe Mckee, Emma van der Slagt, Elisa Gonçalves De Andrade, Hai Lam Nguyen, Penelope A. Young, Marie-Ève Tremblay, Leigh Anne Swayne.
  • DOI: 10.3389/fnana.2021.7224431.
  • Type: Original research article.

Purinergic signaling in nervous system health and disease: Focus on pannexin 1

Pharmacology & Therapeutics

N/A

2021 - 2021

  • Juan C. Sanchez-Arias, Emma Slagt, Haley A. Vecchiarelli, Rebecca C. Candlish, Nicole York, Penelope A. Young, Olga Shevtsova, Afnan Juma, Marie-Ève Tremblay, Leigh Anne Swayne.
  • DOI: 10.1016/j.pharmthera.2021.1078402.
  • Type: Review article.

ATP Triggers Macropinocytosis That Internalizes and Is Regulated by PANX1

bioRxiv

N/A

2020 - 2020

  • Andrew K. J. Boyce, Emma Slagt, Juan C. Sanchez-Arias, Leigh Anne Swayne.
  • DOI: 10.1101/2020.11.19.3890723.
  • Type: Original research article.

Pannexin 1 Regulates Dendritic Protrusion Dynamics in Immature Cortical Neurons

eNeuro

N/A

2020 - 2020

  • Juan C. Sanchez-Arias, Rebecca C. Candlish, Emma Slagt, Leigh Anne Swayne.
  • DOI: 10.1523/ENEURO.0079-20.20204.
  • Type: Original research article.

PANX1 in Inflammation Heats up: New Mechanistic Insights with Implications for Injury and Infection

Cell Calcium

N/A

2020 - 2020

  • Juan C. Sanchez-Arias, Leigh E. Wicki-Stordeur, Rebecca C. Candlish, Emma Slagt, Irina Paci, Praveen P. N. Rao, Brian A. MacVicar, Leigh Anne Swayne.
  • DOI: 10.1016/j.ceca.2020.1022535.
  • Type: Review article.

Consideration of Pannexin 1 Channels in COVID-19 Pathology and Treatment

American Journal of Physiology-Lung Cellular and Molecular Physiology

N/A

2020 - 2020

  • Leigh Anne Swayne, Scott R. Johnstone, Chen Seng Ng, Juan C. Sanchez-Arias, Miranda E. Good, Silvia Penuela, Alexander W. Lohman, Abigail G. Wolpe, Victor E. Laubach, Michael Koval, Brant E. Isakson.
  • DOI: 10.1152/ajplung.00146.20206.
  • Type: Perspective article.

Ankyrin-B p.S646F undergoes increased proteasome degradation and reduces cell viability in the H9c2 rat ventricular cardiomyoblast cell line

Biochemisry & Cell Biology

N/A

2020 - 2020

  • Lena Chen, Catherine S. W. Choi, Juan C. Sanchez-Arias, Laura T. Arbour, Leigh Anne Swayne.
  • DOI: 10.1139/bcb-2019-00827.
  • Type: Original research article.

Ankyrin B and Ankyrin B variants differentially modulate intracellular and surface Cav2.1 levels

Molecular Brain

N/A

2020 - 2020

  • Catherine S. W. Choi, Ivana A. Souza, Juan C. Sanchez-Arias, Gerald W. Zamponi, Laura T. Arbour, Leigh Anne Swayne.
  • DOI: 10.1186/s13041-019-0494-88
  • Type: Original research article.

A novel motif in the proximal C-terminus of Pannexin 1 regulates cell surface localization

Scientific Reports

N/A

2019 - 2019

  • Anna L. Epp, Sarah N. Ebert, Juan C. Sanchez-Arias, Leigh E. Wicki-Stordeur, Andrew K. J. Boyce, Leigh Anne Swayne.
  • DOI: 10.1038/s41598-019-46144-59
  • Type: Original research article.

Exploring the Pannexin 1 interactome: In silico cross-analyses with postsynaptic proteins and neuropsychiatric disorder susceptibility genes

bioRxiv

N/A

2019 - 2019

  • Simona D. Frederiksen, Leigh E. Wicki-Stordeur, Juan C. Sanchez-Arias, Leigh Anne Swayne.
  • DO: 10.1101/80156310
  • Type: Original research article.

Pannexin 1 Regulates Network Ensembles and Dendritic Spine Development in Cortical Neurons

eNeuro

N/A

2019 - 2019

  • Juan C. Sanchez-Arias, Mei Liu, Catherine S. W. Choi, Sarah N. Ebert, Craig E. Brown, Leigh Anne Swayne.
  • DOI: 10.1523/ENEURO.0503-18.201911
  • Type: Original research article.

Probenecid Disrupts a Novel Pannexin 1-Collapsin Response Mediator Protein 2 Interaction and Increases Microtubule Stability

Frontiers in Cellular Neuroscience

N/A

2018 - 2018

  • Xiaoxue Xu, Leigh E. Wicki-Stordeur, Juan C. Sanchez-Arias, Mei Liu, Maria S. Weaver, Catherine S. W. Choi, Leigh A. Swayne.
  • DOI: 10.3389/fncel.2018.0012412
  • Type: Original research article.

Upregulation of inflammatory mediators in the ventricular zone after cortical stroke

PROTEOMICS - Clinical Applications

N/A

2017 - 2017

  • Adrianna N. Gunton, Juan C. Sanchez-Arias, Esther O. Carmona-Wagner, Leigh E. Wicki-Stordeur, Leigh Anne Swayne.
  • DOI: 10.1002/prca.20160009213
  • Type: Original research article.

Perspectives on the role of Pannexin 1 in neural precursor cell biology

Neural Regeneration Research

N/A

2016 - 2016

  • Juan C. Sanchez-Arias, Leigh E. Wicki-Stordeur, Leigh Anne Swayne.
  • DOI: 10.4103/1673-5374.19322114
  • Type: Review article.

What Are Neural Stem Cells, and Why Are They Important?

Frontiers for Young Kids

N/A

2016 - 2016

  • Leigh Anne Swayne, Juan C. Sanchez-Arias, Agbay Andrew, Stephanie M. Willerth.
  • DOI: 10.3389/frym.2016.0002015
  • Type: Review article.

Pannexin 1 Differentially Affects Neural Precursor Cell Maintenance in the Ventricular Zone and Peri-Infarct Cortex

Journal of Neuroscience

N/A

2016 - 2016

  • Leigh E. Wicki-Stordeur, Juan C. Sanchez-Arias, J. Dhaliwal, Esther O. Carmona-Wagner, Valery I. Shestopalov, Diane C. Lagace, Leigh Anne Swayne.
  • DOI: 10.1523/JNEUROSCI.0436-15.201616
  • Type: Original research article.